BBE medium growth is most characteristic of which Gram-negative genus?

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Multiple Choice

BBE medium growth is most characteristic of which Gram-negative genus?

BBE medium is used to isolate bile-resistant anaerobic Gram-negative rods from the gut and to detect esculin hydrolysis. Bile in the medium inhibits many bacteria, while esculin hydrolysis (with ferric citrate indicating the result) produces a dark coloration. The classic growth pattern with esculin hydrolysis in this bile-containing medium is most characteristic of Bacteroides species, especially the Bacteroides fragilis group, which readily hydrolyze esculin in this environment. While other organisms like Prevotella can grow and Neisseria or Escherichia don’t exhibit this specific esculin-darkening trait on BBE, the defining association on this medium is with the genus Bacteroides (Bacterodies).

BBE medium is used to isolate bile-resistant anaerobic Gram-negative rods from the gut and to detect esculin hydrolysis. Bile in the medium inhibits many bacteria, while esculin hydrolysis (with ferric citrate indicating the result) produces a dark coloration. The classic growth pattern with esculin hydrolysis in this bile-containing medium is most characteristic of Bacteroides species, especially the Bacteroides fragilis group, which readily hydrolyze esculin in this environment. While other organisms like Prevotella can grow and Neisseria or Escherichia don’t exhibit this specific esculin-darkening trait on BBE, the defining association on this medium is with the genus Bacteroides (Bacterodies).

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