Which of the following is NOT a common reason for false negatives in PCR testing?

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Multiple Choice

Which of the following is NOT a common reason for false negatives in PCR testing?

PCR relies on amplifying the target genetic material; a false negative happens when the target is present but not detected. Issues like poor specimen quality reduce how much target is available, and substances such as heparin can inhibit the polymerase, preventing amplification. Using the wrong temperatures during the reaction disrupts the steps of denaturation, annealing, and extension, also leading to no amplification. A high organism load, however, means there is plenty of target material to amplify, which makes detection easier, not harder. So having a high organism load is not a common reason for a false negative.

PCR relies on amplifying the target genetic material; a false negative happens when the target is present but not detected. Issues like poor specimen quality reduce how much target is available, and substances such as heparin can inhibit the polymerase, preventing amplification. Using the wrong temperatures during the reaction disrupts the steps of denaturation, annealing, and extension, also leading to no amplification. A high organism load, however, means there is plenty of target material to amplify, which makes detection easier, not harder. So having a high organism load is not a common reason for a false negative.

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