Which organism is non-lactose fermenting, oxidase negative, nitrate negative, and non-motile?

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Multiple Choice

Which organism is non-lactose fermenting, oxidase negative, nitrate negative, and non-motile?

Think about how labs distinguish these bacteria by a combination of four traits. Non-lactose fermentation points to a small group of enterics, including Shigella and Salmonella. Oxidase negative is common to Shigella, Salmonella, and Escherichia coli, but not to Vibrio, which helps rule out Vibrio vulnificus. The non-motile nature is a hallmark of Shigella among the non-lactose fermenters, since Salmonella and E. coli are motile. Finally, the nitrate test tends to be negative for Shigella while the others in this group can reduce nitrate. Putting it all together, the organism that fits non-lactose fermentation, oxidase negative, nitrate negative, and non-motile is Shigella flexneri.

Think about how labs distinguish these bacteria by a combination of four traits. Non-lactose fermentation points to a small group of enterics, including Shigella and Salmonella. Oxidase negative is common to Shigella, Salmonella, and Escherichia coli, but not to Vibrio, which helps rule out Vibrio vulnificus. The non-motile nature is a hallmark of Shigella among the non-lactose fermenters, since Salmonella and E. coli are motile. Finally, the nitrate test tends to be negative for Shigella while the others in this group can reduce nitrate. Putting it all together, the organism that fits non-lactose fermentation, oxidase negative, nitrate negative, and non-motile is Shigella flexneri.

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