Which organism yields a positive Reverse CAMP test?

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Multiple Choice

Which organism yields a positive Reverse CAMP test?

The reverse CAMP test hinges on how certain bacterial toxins interact with the hemolysins produced by Staphylococcus aureus on blood agar, but in the opposite arrangement of the classic CAMP test. In this reverse setup, organisms that have potent phospholipase activity—most notably Clostridium perfringens with its alpha toxin (lecithinase)—interact with S. aureus factors to create a distinctive reversed zone of hemolysis. That interaction yields a positive reverse CAMP result for C. perfringens, distinguishing it from other organisms. Streptococcus agalactiae, which gives the classic CAMP reaction in the standard test due to its CAMP factor, does not produce the same reversed pattern in this setup, so it wouldn’t yield a positive result here. Clostridium difficile and Staphylococcus aureus don’t produce the specific interaction pattern required for a positive reverse CAMP, so they wouldn’t show this reaction either.

The reverse CAMP test hinges on how certain bacterial toxins interact with the hemolysins produced by Staphylococcus aureus on blood agar, but in the opposite arrangement of the classic CAMP test. In this reverse setup, organisms that have potent phospholipase activity—most notably Clostridium perfringens with its alpha toxin (lecithinase)—interact with S. aureus factors to create a distinctive reversed zone of hemolysis. That interaction yields a positive reverse CAMP result for C. perfringens, distinguishing it from other organisms.

Streptococcus agalactiae, which gives the classic CAMP reaction in the standard test due to its CAMP factor, does not produce the same reversed pattern in this setup, so it wouldn’t yield a positive result here. Clostridium difficile and Staphylococcus aureus don’t produce the specific interaction pattern required for a positive reverse CAMP, so they wouldn’t show this reaction either.

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